Various analyte measurements made in automated clinical chemistry analyzers involve optical techniques such as absorbance, reflectance, fluorescence, and turbidimetric and nephelometric detection. On the other hand, ion-selective (ISE) methods are based on a direct potentiometric technique. Which of these methods is the most efficient in measuring analytes?
Good day Ms. Marasigan,
Spectrophotometry, which follows the principle of absorbance, is a method of measuring analyte that is widely used in clinical laboratories. It is a standard and inexpensive technique to measure light absorption or the amount of analyte present in a serum/plasma. The following criteria that makes spectrophotometry an efficient method for measuring analyte are as follows:
Economic value: These instruments offer an excellent value, providing robust operation and low cost of ownership. They require only minor attention to keep running smoothly. Monitoring overall cleanliness and lamp life are the bulk of the maintenance.
Ease of use: Spectrophotometers are fairly straightforward instruments with very few moving parts; they are therefore relatively easy to use and maintain. In addition, many manufacturers provide intuitive software solutions for a satisfying user experience.
Noninvasive: There is no direct manipulation of the material being measured, thus the integrity of the sample is not compromised.
Revealing contaminants: Full-spectrum absorption readings detect additional peaks, revealing the presence of potential sample contaminants. This information may be useful for downstream applications in which the contaminants could be detrimental to the experiment.
Reference:
Geisler (2015). Choosing the Best Detection Method: Absorbance vs. Fluorescence. Retrieved from: https://www.biocompare.com/Bench-Tips/173963-Choosing-the-Best-Detection-Method-Absorbance-vs-Fluorescence/
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