Which gene should I consider when studying the mutation prevalence in sickle cell anemia? What are the restriction enzymes that can be used?

We are trying to detect the prevalent mutation in case of sickle cell anemia in a specific population. We are confused about which gene we should consider as we were unable to find much literature...

10 November 2014 8,671 8 View

Can anyone provide us with the culture of Thermus aquaticus for educational purpose?

We were planning on a starting a study group and a workshop/training on recombinant DNA technology and were thinking of trying to clone the DNA Pol I gene of Thermus aquaticus. We tried isolating...

08 September 2014 9,961 1 View

Is there a protocol for isolation of Large Cardamom Chhirke Virus from infected plant sap under low rcf?

We were thinking of trying to isolate the plant virus in hopes of starting a trial research on production of rabbit poly clonal antibodies against the virus. But we haven't been able to find a...

07 August 2014 7,883 3 View

Can I prepare a single phylogenetic tree using sequences from two different loci?

We have sequenced two different loci (ITS and rbcl) and I was wondering if I could combine both loci and build a single phylogenetic tree.

05 June 2014 3,038 9 View

What are the possible information that can be inferred from ITS and rbcl sequencing?

We sequenced plant DNA samples using ITS, 5.8s and rbcl primers and we were planning on performing a phylogenetic analysis but I wanted to know what other possible information could be inferred...

04 May 2014 4,753 4 View

When performing DNA sequence analysis, how should we treat the gaps?

We performed sequencing of PCR product using a forward and the reverse primer. When the results came out and when we tried aligning the forward sequence with the reverse sequence, multiple gaps...

04 May 2014 1,253 8 View

Why and how does PEG precipitate DNA? Why is it better than isopropanol to reduce the polysaccharide contaminants?

We used to perform isopropanol precipitation of DNA isolated from plant tissues but had a lot of polysaccharides as contaminants and while going through different literatures we found the use of...

02 March 2014 5,232 3 View

How to avoid and exclude DNA fragments, polysacharides and polyphenols during DNA isolation from silica dried leaf samples?

We performed DNA isolation from a silica dried Taxus leaves using a modified CTAB buffer (ammonium acetate and propanol precipitation) as well as PEG precipitation but we could not account for the...

01 February 2014 7,027 20 View

What colour would a leaf take if we avoid green light and grow the plant under blue and red light illuminations only?

I was just wondering if we grow a plant under restricted blue and red light illuminations only, what colour would the leaves show? What percentage of the light would be absorbed and what...

10 November 2013 8,946 3 View

How do I test whether my primer amplifies other genomes than the one it is targeted for?

We ordered a primer for our project. I want to test whether the primer amplifies genes from other organisms too or not. Is there any software to test whether my primer works for other genomes or...

10 November 2013 1,963 6 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View