13 Questions 17 Answers 0 Followers
Questions related from Ashish Bhandari
We are trying to detect the prevalent mutation in case of sickle cell anemia in a specific population. We are confused about which gene we should consider as we were unable to find much literature...
11 November 2014 8,763 8 View
We were planning on a starting a study group and a workshop/training on recombinant DNA technology and were thinking of trying to clone the DNA Pol I gene of Thermus aquaticus. We tried isolating...
09 September 2014 10,037 1 View
We were thinking of trying to isolate the plant virus in hopes of starting a trial research on production of rabbit poly clonal antibodies against the virus. But we haven't been able to find a...
08 August 2014 7,939 3 View
We have sequenced two different loci (ITS and rbcl) and I was wondering if I could combine both loci and build a single phylogenetic tree.
06 June 2014 3,104 9 View
We performed sequencing of PCR product using a forward and the reverse primer. When the results came out and when we tried aligning the forward sequence with the reverse sequence, multiple gaps...
05 May 2014 1,302 8 View
We sequenced plant DNA samples using ITS, 5.8s and rbcl primers and we were planning on performing a phylogenetic analysis but I wanted to know what other possible information could be inferred...
05 May 2014 4,832 4 View
We used to perform isopropanol precipitation of DNA isolated from plant tissues but had a lot of polysaccharides as contaminants and while going through different literatures we found the use of...
03 March 2014 5,312 3 View
We performed DNA isolation from a silica dried Taxus leaves using a modified CTAB buffer (ammonium acetate and propanol precipitation) as well as PEG precipitation but we could not account for the...
02 February 2014 7,142 20 View
We ordered a primer for our project. I want to test whether the primer amplifies genes from other organisms too or not. Is there any software to test whether my primer works for other genomes or...
11 November 2013 2,023 6 View
While trying isolation of thermophiles, we came across a difficulty: when we incubated our sample at 50 degree celsius, almost most of the media dried up. How do we prevent the drying up of...
11 November 2013 6,024 16 View
We were trying to isolate some thermophilic bacteria when the idea suddenly struck us to isolate Thermus aquaticus from hot springs. But before approaching hot spring sources we wanted to try...
11 November 2013 7,693 4 View
I was just wondering if we grow a plant under restricted blue and red light illuminations only, what colour would the leaves show? What percentage of the light would be absorbed and what...
11 November 2013 9,033 3 View
When we tried isolating genomic DNA from buccal swabs, high protein contamination was seen. We followed a simple procedure, using SDS to disrupt the cells and precipitation using ice cold ethanol...
10 October 2013 8,637 2 View
