There are the lesser known antigens of gd T cells especially in viral infection. Is it possible to have a whole blood infection model to expand gd T cells to study their role in infection?
It works best if you seperate PBMNC and incubate with the antigen, culture for 12 days in the presence of IL2 and the do the immunophenotyping using subset specific antibodies.
It may vary among different pathogens, for our virus, its replication mainly restrains in intestinal tissues. We harvest the payer's patches (PP) from infected animals and isolate the bulk lymphocytes and stain for gd TCR. Ex vivo study also works if you culture the lymphocytes from PP for 3 days and do the infection, then look at gd T subsets. That's being said, it depends on whether your virus disseminates to the peripheral or not to establish such model.
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