Getting single peak in LC-MS which is -2 of molecular weight of my compound?
If you are referring to a single fragment on MS, could be a loss of 2 hydrogens to form a double bond. If not MS issue, please give more information.
In ESI/MS, [M + H]- operation peak corresponding to deprotonated analyte molecule is observed
most probably maybe it is contamination check solvent and container.
in addition, the organic compound has monoisotopic C12,H1, O16
Mass significantly less than the average mass.
If there are 3 lines in the molecular ion region (M+, M+2 and M+4) with gaps of 2 m/z units between them, and with peak heights in the ratio of 9:6:1, the compound contains 2 chlorine atoms. The molecular weight might be between 108 1nd 110. Bromine has two isotopes, 79Br and 81Br in an approximately 1:1 ratio (50.5 : 49.5 if you want to be fussy!). That means that a compound containing 1 bromine atom will have two peaks in the molecular ion region, depending on which bromine isotope the molecular ion contains.
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