Pavan P R If you ask "is it possible by chance" - yes, it happens sometime in experiments. If you ask "is there any special method to reproduce such result" - No.
Yes, it is possible to obtain haploid or doubled haploid plants (also known as hexploid or 6x plants) through anther culture of diploid plants, although the frequency of obtaining such plants can vary depending on the species and the specific conditions of the culture.
The basic process of anther culture involves the in vitro culture of anthers (male reproductive structures) on a nutrient medium under controlled conditions. The anthers contain haploid cells, and under the right conditions, these cells can undergo embryogenesis and produce haploid or doubled haploid plants.
In some cases, the haploid plants produced by anther culture can spontaneously double their chromosome number, resulting in doubled haploid plants (6x in the case of diploid plants). This can occur through a process known as spontaneous chromosome doubling or through the application of colchicine or other chromosome-doubling agents.
It is important to note that the production of doubled haploid plants through anther culture is a complex and often unpredictable process, and the success rate can vary greatly depending on the species and the specific conditions used. It may require optimization of the anther culture protocol, including the use of specific media, growth regulators, and environmental conditions, to increase the frequency of doubled haploid plant production.
Bhojwani, S. S., & Razdan, M. K. (1986). Plant tissue culture: theory and practice. Elsevier.
Kasha, Ken J. "Chromosome doubling and recovery of doubled haploid plants." Haploids in crop improvement II (2005): 123-152.
Palmer, C. D., Keller, W. A., Keller, W. A., Kasha, K., & Kasha, K. J. (Eds.). (2005). Haploids in crop improvement II. Springer Science & Business Media.
Ferrie, A. M. R., & Caswell, K. L. (2011). Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell, Tissue and Organ Culture (PCTOC), 104, 301-309.
Dhan Pal Singh, Asheesh K. Singh, Arti Singh,
Chapter 7 - Haploidy and polyploidy in crop improvement,
Editor(s): Dhan Pal Singh, Asheesh K. Singh, Arti Singh,
Plant Breeding and Cultivar Development,
Academic Press,
2021,
Pages 179-192,
ISBN 9780128175637,
https://doi.org/10.1016/B978-0-12-817563-7.00008-8.
(https://www.sciencedirect.com/science/article/pii/B9780128175637000088)
Abstract: Changes in chromosome number, gene mutation, and genetic recombination are important sources of genetic variation, and resulted in evolution of numerous crop species. Doubled haploid (DH) production has been considered as a major factor in reducing the amount of time needed to develop pure lines and inbred lines in crops that are amenable to tissue culture. The major methods of DH production are anther culture, microspore culture, and interspecies or intergeneric crosses. In this chapter, DH production methods and its success in wheat, maize, and barley are described, and advantages/disadvantages are presented. Polyploidy and its usefulness in plant breeding are also covered.
Keywords: Doubled haploid; haploid; anther culture; microspore culture; wheat × maize system; vulgare × bulbosum; chromosome doubling; polyploidy; autopolyploid; allopolyploid
there are many more...
you can also check the following:
N, S. (2020) What is anther culture? history, methods and Protocol, Biology Reader. Available at: https://biologyreader.com/anther-culture.html (Accessed: March 1, 2023).
Pavelek and Griga (2020) Anther culture, Anther Culture - an overview | ScienceDirect Topics. Handbook of Natural Fibres (Second Edition). Available at: https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/anther-culture (Accessed: February 28, 2023).
Gilles, L.M. et al. (2017) Haploid induction in plants, Current Biology. Elsevier. Available at: https://www.cell.com/current-biology/fulltext/S0960-9822(17)30959-4 (Accessed: March 1, 2023).
Wan, Y., Petolino, J. F., & Widholm, J. M. (1989). Efficient production of doubled haploid plants through colchicine treatment of anther-derived maize callus. Theoretical and Applied Genetics, 77, 889-892.
Ebrahimzadeh, H., Soltanloo, H., Shariatpanahi, M. E., Eskandari, A., & Ramezanpour, S. S. (2018). Improved chromosome doubling of parthenogenetic haploid plants of cucumber (Cucumis sativus L.) using colchicine, trifluralin, and oryzalin. Plant Cell, Tissue and Organ Culture (PCTOC), 135, 407-417.
Roberts and Blake (2003) Chromosome doubling, Chromosome Doubling - an overview | ScienceDirect Topics. Available at: https://www.sciencedirect.com/topics/immunology-and-microbiology/chromosome-doubling (Accessed: March 1, 2023).
Rajcan, I., Boersma, J. G., & Shaw, E. J. Plant Genetics Techniques: Plant Breeders Toolbox. Comprehensive Biotechnology. 4th; 2011.
Yes we can, first we have to cross diploid with tetraploid - this is how we get sterile triploid - and then we have using colchicine double the chromosome number - this is how we can get hexaploid progeny.
Haploid, diploid, tripod, polyploid explained: https://youtu.be/nVH-ajrVoHQ
Aneuploid, Euploid, Polyploid, Tetraploid: https://youtu.be/U0X-nB6vnE0
Polyploidy explained with examples: https://youtu.be/vXQwHEfP1VM
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