What may be the possible reasons for getting a Ct value in case of non template control during qRT PCR?

In my lab we carried out qRT PCR for checking the efficiency of the primers. The results came fine for dilutions, unfortunately for non template control also we got good expressions. Inorder to...

14 January 2021 9,425 5 View

Could u help with different techniques installed across the world to prevent jelly fish ingress problems in sea water intake syts desalination plant?

jelly fish ingress is a major problem affecting the sea water intake systems leading to plant outages in power plants and desalination plants. can anyone through some insight on installed and...

15 December 2020 6,643 4 View

What stage of the rice plants is ideal for rhizosphere soil sampling?

I want to isolate PGPR from rice rhizosphere (flooded conditions). Not sure when to collect samples. Concentrating on acidic soils of South India. The seeds are being sown now for the second...

27 May 2020 9,516 1 View

Which Zeolite can be prepared under atmospheric pressure without using any template?

Which Zeolites can be prepared under atmospheric pressure without using any template (organic or other) using only aluminium source and silica source ?

24 December 2019 6,356 3 View

Why is there a change in EFL measurement in liquid medium?

Hello Everyone, This is regarding the EFL change of a biconvex lens. Can someone explain to me the reason for a change in the measurement of a boconvex lens in water when measured with a time gap...

07 August 2019 7,610 1 View

What is the effect of Si/Al ratio on behavior of Portland cement concrete or high performance concretes (NOT on Geopolymer Concrete)?

It is a known fact that the Si/Al ratio of the cementitious matrix influences the behavior of the geopolymer concrete. However, I would like to know about the effect of the Si/Al ratio of the...

21 May 2019 8,082 7 View

Dissappearing peaks in rietveld refinement?

I've been trying to obtain the cell parameters of CeVO4 and SmVO4 from rietveld refinement of XRD data. However the peaks seem to disappear after refining U, V, W parameters in Full-Prof software...

11 April 2019 7,377 3 View

How to identify optically thin Cirrus Clouds in historical satellite images acquired with Passive Remote Sensing satellite sensors?

Unlike Optically thick clouds, Cirrus Clouds are thin, high altitude clouds formed in the upper troposphere layer of the Earth's Atmosphere. These Cirrus Clouds are not easily identifiable in the...

23 March 2019 1,765 3 View

How to do coulomb counting in lead acid battery to estimate Soc?

I am doing a project based on demand side management of DC grid system by Soc of battery. Currently I am stuck in Soc estimation practically in lab using coulomb counting. Does anybody knows,...

19 March 2019 702 3 View

Low Chronbach's alpha. Reason?

I have translated a questionnaire for research purposes and in spss, the internal consistency reliability is coming low. The original questionnaire has good reliability. what could be the reason...

20 October 2018 442 3 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

Why CHi^2 is reducing if I divide the intensity by a constant factor?

I was refining my XRD data in FullProf. Highest intensity was 400000. The fitting was good but still the Chi^2 value is very high (0.174E+04) I divided the intensity by some constant factor (say...

17 February 2021 3,383 5 View

Is it possible to do Reitveld Refinement on single crystal XRD data?

I tried to do single-crystal Rietveld Refinement on my single-crystal XRD data using the option "refinement on single-crystal data/ integrated intensity data" in Fullprof but it is giving error...

30 January 2021 7,813 3 View

How to take into account the high resolution of the x-ray difractometer?

At small angles or in case high collimation, the peaks become anisotropic and with a Lorentz addition. The resolution describes correctly only with adding Lorentz and anisatropy parameters. It...

26 January 2021 5,829 4 View

Can someone advise me to do Rietveld Refinement of the material whose Crystallographic Information Framework (CIF) file is not available?

10 October 2020 7,411 9 View

How to fix the refinement on Fullprof?

I have an XRD pattern of malachite phase in a range of 5-50 degree in 2 theta. After that, I use MID-JADE software to refine the pattern and it shows a good refinement with low R-value and GOF...

03 October 2020 3,613 8 View

How is it possible to simulate or model particle morphology using X-ray powder diffraction?

Hello everyone! I suppose the XRD of sample LaPO4 has some anisotropic line broadening along [h00]. How could I model the shape of particle using WPPF method or something else? Diffraction...

21 September 2020 1,823 3 View

Can someone help me with a standard Silicon Rietveld Refinement in GSAS II?

15 September 2020 3,085 7 View

What's the acceptable value of _refine_ls_shift/su_max for Rietveld refinement?

Hi, my background is in single-crystal X-ray diffraction. Now, I am carrying out the so-called Rietveld refinements in GSAS-II and am getting quite large _refine_ls_shift/su_max values (ranging...

01 September 2020 7,012 2 View