What may be the possible reasons for getting a Ct value in case of non template control during qRT PCR?

In my lab we carried out qRT PCR for checking the efficiency of the primers. The results came fine for dilutions, unfortunately for non template control also we got good expressions. Inorder to...

14 January 2021 9,425 5 View

Could u help with different techniques installed across the world to prevent jelly fish ingress problems in sea water intake syts desalination plant?

jelly fish ingress is a major problem affecting the sea water intake systems leading to plant outages in power plants and desalination plants. can anyone through some insight on installed and...

15 December 2020 6,643 4 View

Do you know any free or low-cost software/licenses of programming assistance for phone-based task that would allow you to design a Fitts task?

In these troubled times, as part of a study project I would like to develop a phone-based Fitts task with the tactile response modality. However, without special help programming a task in JAVA...

30 November 2020 9,098 3 View

Effect of heat and maltose on maltose binding protein fusion apparent molecular weight?

22 November 2020 648 3 View

Thermochemistry for a Multigas System?

I'm familiar with how concepts such as the latent heat of fusion and vaporization work for a system consisting of a single phase-changing gas. q = mH, q=mc dt, all that fun stuff. I'm not...

27 September 2020 4,985 1 View

Where can I download the expanded MRC Psycholinguistic Database with Imageability Ratings?

I've been reading a journal entitled 'Automatic Expansion of the MRC Psycholinguistic Database Imageability Ratings ' and would like to use the expanded dataset for my research. Does anyone know...

08 July 2020 2,343 1 View

Can you help for the latex external bibliography in IET journal?

I am having a problem for the latex external bibliography in the IET journal. The solution found in google does not work. The compiler can not find \bibliographystyle{iet} in the cta-author.cls.

30 May 2020 4,062 6 View

What stage of the rice plants is ideal for rhizosphere soil sampling?

I want to isolate PGPR from rice rhizosphere (flooded conditions). Not sure when to collect samples. Concentrating on acidic soils of South India. The seeds are being sown now for the second...

27 May 2020 9,516 1 View

Run Coulomb (seismic stress modeling) on Octave, possible? Or an equivalent?

I'm working on earthquakes energy modelizations around faults under Windows 10. Here's the problem: I need Coulomb to modelize shear / normal / coulomb stress, but it isn't (correctly) working on...

22 March 2020 1,465 2 View

Which Zeolite can be prepared under atmospheric pressure without using any template?

Which Zeolites can be prepared under atmospheric pressure without using any template (organic or other) using only aluminium source and silica source ?

24 December 2019 6,356 3 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View