Is it possible to extract miRNAs manually from cells? If yes. then what is the protocol to isolate them?

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Samuel Gebremedhn

It is possible to extract miRNAs from cells, body fluids (plasma/serum) and tissues samples. In our laboratory we use miRNAs enriched total RNA extraction using miRNeasy mini kit (Qiagen) and we usually get good reults.

Vladimir Mulens-Arias

You could use mirVANA miRNA Isolation Kit from Ambion as well. It usually works great.