How to do interdisciplinary research combining literature and visual arts?

I am a first-year PhD student who would like to hear about other researchers' experience related to interdisciplinarity within the humanities, especially those combining both text and visual...

26 May 2020 2,889 37 View

What is the best method to store sectioned FFPE slides for long term storage?

I will be having FFPE blocks cut onto slides, I would like to know what would be the best method to store slides for long term storage. I don't want the slides to go bad. I am worried about...

16 January 2019 9,208 2 View

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28 September 2018 7,258 4 View

What are good control tissues for a MDSC Immunohistochemistry panel?

Hi Everyone, I am currently working on a human MDSC immunohistochemistry panel using CD33, CD11b and HLA-DR. I need to find good control tissue. I tried to use normal brain and spleen tissue...

23 September 2018 4,882 3 View

What are good control tissues for a MDSC Immunohistochemistry panel?

Hi Everyone, I am currently working on a human MDSC immunohistochemistry panel using CD33, CD11b and HLA-DR. I need to find good control tissue. I tried to use normal brain and spleen tissue...

08 September 2018 7,464 3 View

Can anyone recommend a sweetener that increases salivary secretion without disturbing original blood glucose levels?

To collect salivary samples (about 1 ml) from spider monkeys. The samples will be analyzed to determine cortisol, melatonin, serotonin and testosterone through ELISA tests. Thanks for any...

06 July 2018 3,605 3 View

Does anyone know how to calculate the number of peptide bonds of a protein after hydrolysis?

Need that calculation to obtain the degree of hydrolysis.

07 August 2017 6,463 0 View

Which ANTS/DPX concentration should I use to see solute leakage from liposomes?

I want to see how ANTS fluorescence in liposomes changes when adding triton.My idea is to encapsulate ANTS and its quencher DPX in the same liposome so that when adding triton fluorescence signal...

27 July 2017 9,640 1 View

How can I avoid nanoerythrosomes aggregation?

15 March 2017 10,022 6 View

Can orange/blue loading dye or green master mix can cause anomalous bands in my electrophoresis from a purified PCR product?

I am trying to purified a PCR product from gel extraction (2% agarose), for the purification I used PureLink Quick gel extraction and PCR purification combo kit (Invitrogen). And for the PCR...

28 September 2016 9,012 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View