we gfp transformed Mycobacteria in soil degradation and used a MER promoter to 'light the organisms' up on agar cultures of the soil to follow progress. If you insert the gfp constituitively it is likely to cause an metabolic burden on the organism and so impair competition. I suggest you look at promoters that could be applied 'in situ' to induce gfp expression. Obviously Mercury is not a good one - but perhaps other less harmful promoters (kan?) could be used?