Which FEM software should I use to run a thermal analysis with quiet and inactive elements ?

I read in some articles that it has already been done with Netfabb but I'm searching for a free software.

07 December 2020 6,759 3 View

Do you know if, in your country, some specific measures regarding young people have been put in place since the beginning of the pandemic?

Dear colleagues, I have been asked to write an editorial about young adults facing the pandemic and measures to be taken to mitigate social risks (in France). I am looking for some international...

14 October 2020 8,202 9 View

N min from frozen soil samples?

22 June 2020 6,262 3 View

How to classify AI-based technologies used in health care settings?

Hello everyone! I am supporting health care managers in the identification and classification of all AI-based health care technologies present in their hospitals (technologies for clinical,...

01 January 2020 3,431 3 View

What are the best journals to publish on Social Innovation?

27 November 2018 9,395 4 View

cDNA sequencing directly from purified PCR products or after cloning?

Could someone tell me the pros and cons of each, regarding the main downstream applications? Thank you!

17 September 2017 3,410 10 View

Does the use of RNAse inhibitor is mandatory in Reverse-Transcription experiments?

I'm using the Sensiscript Reverse Transcription kit from Qiagen which recommends that but I've also been told that it's not necessary as long as I work in a RNAse free environment thanks to...

19 April 2017 927 4 View

How to estimate and remove physiological noise from fMRI resting state dataset?

Hi, We are working on some resting state functional fMRI dataset that have not been acquired in our laboratory. Unfortunately, we did not get physiological information with the raw data. Does...

02 March 2017 5,869 1 View

How to use Partial Least Squared Regression with R, considering that Y is a matrix in Y = BX + E ?

(The example in R uses vectors to illustrate Y, not matrix.) I understand with the R exemple using the yarn database, that when using plsr : regpls

22 February 2017 5,296 3 View

Has anybody ever seen these "waves" on a native gel ?

I'm working on proteasomal activity on C.elegans. I extracted worms with lysis buffer containing :20mM tris, 5mM MgCl2, 1mM DTT, 1mM ATP, 10% glycerol, 0,05 NP40. I ran a native gel...

23 November 2016 9,029 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View