Which FEM software should I use to run a thermal analysis with quiet and inactive elements ?

I read in some articles that it has already been done with Netfabb but I'm searching for a free software.

07 December 2020 6,759 3 View

Do you know if, in your country, some specific measures regarding young people have been put in place since the beginning of the pandemic?

Dear colleagues, I have been asked to write an editorial about young adults facing the pandemic and measures to be taken to mitigate social risks (in France). I am looking for some international...

14 October 2020 8,202 9 View

N min from frozen soil samples?

22 June 2020 6,262 3 View

How to classify AI-based technologies used in health care settings?

Hello everyone! I am supporting health care managers in the identification and classification of all AI-based health care technologies present in their hospitals (technologies for clinical,...

01 January 2020 3,431 3 View

What are the best journals to publish on Social Innovation?

27 November 2018 9,395 4 View

cDNA sequencing directly from purified PCR products or after cloning?

Could someone tell me the pros and cons of each, regarding the main downstream applications? Thank you!

17 September 2017 3,410 10 View

Does the use of RNAse inhibitor is mandatory in Reverse-Transcription experiments?

I'm using the Sensiscript Reverse Transcription kit from Qiagen which recommends that but I've also been told that it's not necessary as long as I work in a RNAse free environment thanks to...

19 April 2017 927 4 View

How to estimate and remove physiological noise from fMRI resting state dataset?

Hi, We are working on some resting state functional fMRI dataset that have not been acquired in our laboratory. Unfortunately, we did not get physiological information with the raw data. Does...

02 March 2017 5,869 1 View

How to use Partial Least Squared Regression with R, considering that Y is a matrix in Y = BX + E ?

(The example in R uses vectors to illustrate Y, not matrix.) I understand with the R exemple using the yarn database, that when using plsr : regpls

22 February 2017 5,296 3 View

Has anybody ever seen these "waves" on a native gel ?

I'm working on proteasomal activity on C.elegans. I extracted worms with lysis buffer containing :20mM tris, 5mM MgCl2, 1mM DTT, 1mM ATP, 10% glycerol, 0,05 NP40. I ran a native gel...

23 November 2016 9,029 4 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is the logarithmic transformation a necessary step in time series analysis ?

Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...

15 February 2021 5,655 14 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Mixed ANOVA with some normal and some non-normal data?

I am looking to run a 2x5 Mixed ANOVA to examine Sensory Processing Sensitivity in Highly Sensitive People (HSP) and Non_HSP. My Between-Subjects factor is HSP and Non-HSP. All participants tested...

11 February 2021 1,445 6 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

After transformation of Dh5alpha competent cells I found a cloudy white ppt before plating. Can anyone explain this?

After transformation of Dh5alpha cells by heat shock I grew them at 37 in 200microlitre Terrific Broth (because I had it at hand) and then before plating I found cloudy white ppt in the cell...

03 February 2021 7,867 3 View