Which FEM software should I use to run a thermal analysis with quiet and inactive elements ?

I read in some articles that it has already been done with Netfabb but I'm searching for a free software.

07 December 2020 6,759 3 View

Do you know if, in your country, some specific measures regarding young people have been put in place since the beginning of the pandemic?

Dear colleagues, I have been asked to write an editorial about young adults facing the pandemic and measures to be taken to mitigate social risks (in France). I am looking for some international...

14 October 2020 8,202 9 View

N min from frozen soil samples?

22 June 2020 6,262 3 View

How to classify AI-based technologies used in health care settings?

Hello everyone! I am supporting health care managers in the identification and classification of all AI-based health care technologies present in their hospitals (technologies for clinical,...

01 January 2020 3,431 3 View

What are the best journals to publish on Social Innovation?

27 November 2018 9,395 4 View

cDNA sequencing directly from purified PCR products or after cloning?

Could someone tell me the pros and cons of each, regarding the main downstream applications? Thank you!

17 September 2017 3,410 10 View

cDNA sequencing directly from purified PCR products or after cloning?

Could someone tell me the pros and cons of each, regarding the main downstream applications? Thank you!

08 September 2017 229 11 View

Does the use of RNAse inhibitor is mandatory in Reverse-Transcription experiments?

I'm using the Sensiscript Reverse Transcription kit from Qiagen which recommends that but I've also been told that it's not necessary as long as I work in a RNAse free environment thanks to...

19 April 2017 927 4 View

How to estimate and remove physiological noise from fMRI resting state dataset?

Hi, We are working on some resting state functional fMRI dataset that have not been acquired in our laboratory. Unfortunately, we did not get physiological information with the raw data. Does...

02 March 2017 5,869 1 View

How to use Partial Least Squared Regression with R, considering that Y is a matrix in Y = BX + E ?

(The example in R uses vectors to illustrate Y, not matrix.) I understand with the R exemple using the yarn database, that when using plsr : regpls

22 February 2017 5,296 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View