Maybe at some specific conditions, no wind resistance can be achived?
Possibly, if Chi has no wind resistance. I haven't seen that tested. Your Astral projection also may not have wind resistance.
In swat, epic, or any other models to calculate inorganic phosphorus cycle, why inorganic phosphorus in active pool can be transfered to stable pool, but inorganic phosphorus in labile pool can...
28 February 2024 1,530 4 View
I am docking a protein structure at different pHs. After obtaining the PDB structure, I protonate the protein at different pHs using pdb2pqr, download the structure, then use Autodock vina to dock...
07 February 2024 6,446 0 View
Hi all, We have a lentiviral vector with a gene of interest that we would like to express. There is no requirement for stable cell line construction, so we will only be transfecting transiently....
29 November 2023 2,802 6 View
I have a spectrum CSV file and I have tried using the spliceCorrection function in R package "prospect" to fix it, but the result is not good. Can anyone please guide me on a method (Software) to...
24 September 2023 1,392 0 View
In most protocols, 50% methanol is used for protein A regeneration, followed by storage, usually in water/buffer/20% ethanol. In my experience, it is critical to apply a 50% methanol wash so the...
29 August 2023 2,511 3 View
Hi all, I am trying to produce an antibody from a plasmid in Freestyle CHOS cells. The plasmid is a HC/furin/p2A/LC vector. The cells were transiently transfected during the exponential phase and...
12 July 2023 2,425 5 View
For a project we needed to use a specific device (Bio-Rad Gene pulser) to electroporate mammalian cells. We set up the instrument with the following settings: Voltage = 1.9kV (we had to use this...
02 July 2023 7,753 3 View
Hello all, I recently started to use peptides for cellular studies. Unfortunately, when I was designing the peptides I did not have a FITC tag to them (the peptide was synthesized by an external...
31 May 2023 1,689 3 View
Hello, We have been trying to insert peptide tags (
07 March 2023 425 6 View
Hello all, I'm using Discovery studio 2019 to dock protein-protein, while "An error was encountered during execution of ViewResults.ds_pl.", then the dock failed. As it works quite well with the...
30 November 2022 829 4 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I saw a paper(PHAN V-T, ZHANG X, LI Y, et al. Microscale modeling of creep deformation and rupture in Nickel-based superalloy IN 617 at high temperature [J]. Mechanics of Materials, 2017, 114:...
16 July 2024 8,436 0 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...
10 July 2024 7,457 1 View
If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...
03 July 2024 2,371 1 View
We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...
01 July 2024 2,686 2 View
thanks
25 June 2024 4,667 0 View
Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...
23 June 2024 4,763 0 View
23 June 2024 6,583 2 View
For additional information, I am a master's student looking for a LCA software to do my thesis. So a less expensive one is my first priority, but if it is not able to help me with a clarity...
18 June 2024 8,376 3 View