Hi all,

As an update, I think it worked. In the recent electroporation parts of the cell became slimy - some cells are probably lysed. And I did see some protein expression, although not incredibly much.

It's great that you're starting to use the Bio-Rad Gene Pulser for electroporation experiments. Based on the information you've provided, let's address your concerns and provide some advice:

It's important to keep in mind that successful electroporation can be influenced by various factors, including cell type, cell density, voltage, capacitance, resistance, and the medium used.

Chandni Hussain

Thank you for sharing your insights!