A. Asri

3 Questions 7 Answers 0 Followers

Questions related from A. Asri

Is it possible that the calculated LOQ is smaller than LOD in ELISA?

I calculated LOQ and LOD for ELISA but it seems that the LOQ smaller than LOD. According to EPA (1996) and Armbruster & Pry (2008), LOQ cannot be lower than LOD. Where did I could possibly go...

04 April 2019 8,231 4 View

Is it okay if I've done triplicates for samples in 3 ELISA analysis and then I want to proceed with duplicates for the next analysis?

If it's not okay, why?

09 September 2018 7,620 5 View

In ELISA, do I have to subtract the absorbance values of samples with the absorbance values of field blanks?

I am confused whether to subtract the absorbance values of samples with the absorbance values of field blanks or do I subtract those after obtaining the concentration?

09 September 2018 1,779 6 View