Additional bands on Western blot after protein overexpression? What are the additional products?

Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...

26 November 2020 5,080 4 View

Has anyone pooled libraries from ChIP and RNA experiments in one lane for sequencing?

I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!

21 July 2020 1,846 4 View

Is any requirement to the compressed air clearness to use with the laser optics ?

I understand that the compressed air for the laser optics purge (first step of cleaning) such as mirrors or lenses should be "clean", but does anybody can explain me what really does it mean...

01 April 2019 8,021 5 View

Can anybody recommend me a company to buy graphite target useful for PLD technique?

I would like to buy graphite target for further DLC films creation by PLD technique, does anybody have some good experience with company offering it in terms of reliability and good...

14 March 2019 8,314 3 View

Source of DiFi cell line

Do you know where can I buy DiFi cell line? There are not available in ATCC, ECACC or DSMZ.

20 July 2017 3,772 1 View

Can I measure expression of MIR genes on Illumina BeadChip if I prepare cRNA using Ambion Total Prep Kit (based on oligodT primers)?

Because list of probes on the BeadChip contains some of MIR genes (i.e. MIR1-2, MIR1200, MIR1202, MIR1203 etc.) however, as I remember correctly - the mature microRNAs don't contain the polyA...

19 June 2016 2,867 3 View

Has anyone investigated the influence of the head coil type on resting state data?

What do you think about using 8-channel head array coil?

04 January 2014 8,779 2 View

Is there any GC-ECD in NUIG that can be adapt to measure N2O in a gas phase?

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26 November 2013 2,205 1 View

Role of Clostridium genus in gut brain axis. Investigation of neural connectivity loss in Autism Spectrum Disorder (ASD), nosology, treatment and ther

Dear fellows, I am currently working around specified subject. As part of my investigation I recruit volunteers, able to provide faecal tests results, suitable for ASD patients and control....

01 January 1970 6,630 3 View

Drama on the stage vs. new philology

I'm analysing the relation between the work with literary drama on the stage in the theatre and the theory of new philology (medieval texts). Do you have an example for it in the contemporary...

01 January 1970 8,329 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View