Is it possible for one carrier (bats/rats) to be infected by more both saprophytic and intermediate Leptospira species?

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Yann Gomard

Dear Claudia Yeong,

I have no experience on saprophytic and intermediate Leptospira.

On what organs/samples you work? serum?...

Yann

Martin Bartas

Hello Claudia, why not? see this article:

Article Leptospira and Bats: Story of an Emerging Friendship

Claudia Yeong

bat urine samples. cultured for 12 weeks in EMJH media.

Bruno Miotto

I suggest you to sequence both amplicons to confirm the possibility of co-infection

Felipe Fornazari

Good question. I've never read about it, but it may be possible.

Cyrille Goarant

Co-infections actually occur. However, saprophytic leptospires (the L. biflexa cluster) are unable to infect mammals. I am afraid there is either one non-specific PCR product (quite frequent with 16S rRNA targets)... or a contamination issue. Sequencing both products as suggested will help understand what is goiing on here!