Claudia Yeong

3 Questions 3 Answers 0 Followers

Questions related from Claudia Yeong

Phenol-chloroform-isoamyl problem?

This is my first time using commercialised PCI (25:24:1) solution. After adding equal amount of PCI into my supernatant, two of my samples did not form two layers as they should be. After...

09 September 2019 4,950 3 View

Why PCR results and BLAST analysis results are different for this leptospira isolate?

During a conventional PCR amplification, an isolate was found to be positive intermediate using the primer 16S rRNA but was negative towards LipL32 primer. However when the DNA sequences was...

05 May 2018 6,435 4 View

Is it possible for one carrier (bats/rats) to be infected by more both saprophytic and intermediate Leptospira species?

Using a specific primers for rrs gene (saprophytic) and 16S rRNA(intermediate) during a conventional PCR, i obtain bands for each saprophytic(240bp) and intermediate(331bp) using the same DNA...

04 April 2018 8,177 6 View