19 Questions 94 Answers 0 Followers
Questions related from Martin Bartas
Hello, what is currently the best de novo motif/domain search tool or workflow, if we start from user provided set of protein sequences in FASTA? I have used MEME suite (MEME, DREME, GLAM2) for...
07 July 2018 1,815 5 View
Recently I have read interesting paper " The triplet genetic code had a doublet predecessor". Patel A. The triplet genetic code had a doublet predecessor.... What do you think about this...
06 June 2018 8,581 6 View
Hello, I would like to ask what is in your opinion best user friendly tool for designing of universal primers - primers which will amplify target gene of unknown nucleotide sequence (but its...
05 May 2018 7,985 3 View
Hello, I am wondering if could be possible to align set of protein sequences (for example 100 protein sequences) each to each by any user friendly way. I.e. sequence no. 1 with the sequence no....
05 May 2018 8,975 11 View
Hello, I would like to ask if somebody know user friendly way to blast query sequence (protein or nucleotide) against custom (user-defined) database of sequences. Ideally to work on Windows...
05 May 2018 5,262 4 View
Hello, I am interested in obtaining set of all human proteins with the DNA binding ability in FASTA format in one step. Downloading one by one is very tedious for me :-D Do you know any easy...
05 May 2018 4,551 5 View
Hello, please, is there any user-friendly tool like ProtParam which enables multiple queries (multiple gene symbols/other identifiers or fasta sequences)? ProtParam is super tool, but...
05 May 2018 7,201 7 View
I am not able to find it on the internet, nor in supporting information from manufacturer. It would greatly help me in my research... Thanks
05 May 2018 5,969 4 View
Hello, how to randomly sample set of protein sequences from large file (for example I have defined set of 20 000 protein sequences and would like to sample 200 sequences). Any user-friendly...
05 May 2018 2,180 9 View
Hello, could someone recommend the best website with the powerpoint scientific poster templates to download? :-) requirements: great choice of sizes (inches or cm) cool and creative designs free...
05 May 2018 984 0 View
Hello, I am wondering what is the best method for searching very remote homologs of human proteins. For example, if I am interested in protein XY, I would download all protein sequences of XY...
04 April 2018 9,389 2 View
Hello all, I am wondering if there is any simple way to isolate intact (medium or high quality) total RNA from selected zoo animals. What is the best material for this? Fresh saliva, droppings,...
04 April 2018 10,008 2 View
Hello colleagues, I am wondering if it is possible to solve G-quadruplex structure by cryoEM technique...Please, do you have some experiences with it? Because to my best of knowledge, there are...
01 January 1970 8,175 5 View
Hello, is there any user-friendly bioinformatic tool to obtain last common ancestor of two different organisms and estimated age of their separation? E. g., I would like to know the last common...
01 January 1970 6,955 8 View
Hello all, what is your suggestion for choosing the best reference gene for qPCR in higher plants? My experiment is focused on different gene expression under various irradiance (only green...
01 January 1970 1,151 7 View
Dear colleagues, has somebody any tip to minimize plasmid multimerisation during growth of bacterial culture (E. coli, high-copy plasmid Bluescript). I would like to isolate whole plasmid only in...
01 January 1970 4,029 4 View
Hello all, I would like to ask if someone know some user-friendly tool which is able to predict and visualize domains in my protein alignment. I have long protein alignment (over 1000 aa) and I...
01 January 1970 4,858 6 View
Hello, I have list of app. 10000 organisms and want to gain taxonomy information for them (phylum, class, order,...) into some tabular file. I know NCBI Taxonomy browse...
01 January 1970 2,963 2 View
Hello, colleagues, I have a lot of RNAseq data and I am wondering if there is currently some reference workflow or guidelines, something like the famous MIQE guidelines for qPCR. What is your...
01 January 1970 9,610 9 View
