Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

What is wrong with my western blot transfer system? (smearing is occured)?

I'm now doing western blotting for several proteins. but transfer is not perfectly happened. bands are looks like smeared or smudged. And it seems to the lower MW bands are more affected. what did...

06 December 2020 9,153 5 View

How to calculate bulk density, porosity, and void ratio of soil?

If the particle diameter is 2 mm and the particle density is 2.5 g/cm, what is the bulk density, porosity, and void ratio of this soil? I had known that the formula to calculate them. The...

11 October 2020 3,466 3 View

How can I check the deparaffinization during IHC protocol?

Hello. I am a beginner researcher experimenting with IHC for the first time. I would like to know how to make sure it is completely deparaffinized when it is fixed with paraffin and removed with...

22 March 2020 7,795 3 View

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

02 March 2020 3,982 6 View

How to make simulated body fluid using glycerol and ethanol?

Hi. I'm trying to find the method making simulated body fluid using gylcerol and ethanol, but I cannot find it. (I want to make the solution that has similar surface tension and viscosity of human...

05 November 2019 9,051 1 View

How many cells do I need to get a pure plasma membrane? Do you have a kit?

The purpose of this study is to obtain plasma membranes from adherent cells by means of ultracentrifuge. The problem with the experiment is that the yield is too low when obtaining plasma...

01 October 2019 3,375 3 View

What is the difference between PET film and biaxially oriented PET film?

Hi. I'm trying to make LIS(Liquid Impregnated Surfaces) using PET film, so I was looking for the appropriate film product. Then I found biaxially oriented PET film, and I want to know if there is...

22 July 2019 329 1 View

Can I use multilevel regression with skewed count data?

I am studying neighbourhood effects on antisocial behaviour using multilevel modelling. The dependent variable, antisocial behaviour is a count data which varies between 0 and 10 which is a skewed...

28 November 2018 8,633 7 View

In sandwich ELISA in human serum, how can I reduce matrix effects and background?

Hello, I'm setting sandwich ELISA to measure amount of target protein in human serum. I have recombinant protein and checked 2 kinds of antibodes(different epitope) were suitable to perform...

29 March 2018 8,728 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

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02 March 2021 3,060 3 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View