Is there any instrument or method that can quantify Potassium diformate and Sodium formate and formic acid from mixture of them? It would be better to do it without dissolving them in any solvent
Hello, Dear! Generally, I used CHARMM36 + cgenff force field for simulaiton. But,, due to certain parts of lignad, force field cannot be generated using Cgenff. Can I use different force field...
10 July 2024 9,513 3 View
Dear Researchers, I'm trying to develop the quantification of endogenous peptide in tissue using IDMS as the primary method of characterization. I performed analysis for the calibration curve of...
17 June 2024 9,797 0 View
Just revived a sw480 cells from a 2019 batch. Was normal on the first 2 days. After 5 day, the culture media became cloudy, and lot of suspicious white dots. Is this yeast contamination, as I...
21 April 2024 5,726 1 View
I'm trying to analyze Ti alloy containg α″ martensite phase with orthorhombic structure, but there is no phase data file for indexing α″ phase in the database. So I created phase structure data...
12 December 2023 3,317 1 View
Hi I created a 0.1M Li2S4 solution in a DOL:DME solvent mixture following these steps: first dissolving sulfur (S) in a DOL:DME (v/v) solution and then adding Li2S. The solution was stirred at...
06 December 2023 2,282 0 View
why poly lactic acid doesn't have crystallization point in DSC
03 December 2023 5,876 1 View
I'm checking free-floating IHC protocol for my research using mouse brain sample. I've never performed free-floating IHC before, so I am checking protocol deeply. In protocol during several...
21 November 2023 8,800 0 View
I plated HepG2 into a 96-well plate using EMEM containing 10% FBS and 0.1% antibiotic. After 24 hours, when treating with doxorubicin, should I use media that has 10% FBS or FBS free media? Also,...
11 November 2023 412 1 View
How can I create a new doi before I uploead a new text to researchGate, so that I have the chance to include this information into the pdf file?
09 August 2023 952 0 View
I just published a new paper on "Proceedings of the National Institute of Ecology of the Republic of Korea" and try to upload the file on ResearchGate. But I cannot find the journal of this....
04 August 2023 2,923 3 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View