Is it okay to freeze aliquots of cell culture medium and thaw for use when needed?

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Eva Nagyova Popular answer

We used frozen media . See our paper Nagyova et al. 2000

Sham ashok Patil

yes...i used to thaw FBS (fetal bovine serum), and thaw it whenever needed..it never affected my testings.

Eva Nagyova

Mohammad Reza Khorramizadeh

Amog,

I don not recommend frequent cycles of freeze and thaw for culture media, supplements, and feeder. Make working aliquots and freeze them, instead. When thawing, remember to let thermally equilibrate, before use. Good luck!