Compare with single transfection of one plasmid, co-transfection with control siRNA or target siRNA both severely reduced the protein expression of the co-transfected plasmid. Is it normal? What is the problem?
I only used one plasmid in my experiment. The three groups are 1.plasmid only; 2, plasmid+consiRNA; 3, plasmid+target siRNA. I found that the protein level encoded by the plasmid severely reduced in the group 2 and 3, compared with group one.