Chengdong Wang

4 Questions 7 Answers 0 Followers

Questions related from Chengdong Wang

How to dissolve the alcian blue?

I want to do cartilage staining on Xenopus embryos (st 45). Following the protocol, I tried to dissolve 4 mg alcian blue in 7 ml ethanol plus 3 ml acetic acid. I found alcian blue powder is very...

10 March 2021 4,291 2 View

Is it normal that co-transfection of siRNA and plasmid reduces the expression of plasmid?

Compare with single transfection of one plasmid, co-transfection with control siRNA or target siRNA both severely reduced the protein expression of the co-transfected plasmid. Is it normal? What...

22 October 2020 1,557 3 View

Where can I order the chemical HA15?

Could anyone tell me where I can order HA15 (modified TZD) used in the following research to treat melanoma cell?

05 October 2016 8,884 3 View

Is GLP1r function affected by adding myc-tags to the C-terminal?

I am studying the function of GLP1r, a GPCR. I want to add 6 myc tags to the C terminal of GLP1r. Can anyone give some information about whether this will affect the function of GLP1r?

08 April 2016 3,407 2 View