I have a HPTLC plate but Rf value was initially not considered. How can I justify my findings? Will be the fluorescent band of the sample which matches with the standard is more than enough?
Rd value is the characteristic property of a given substance alike a fluorescence pattern. The later one too should do if it is in congruence with the reference but if it shifts to higher or lower wavelength then necessary justification for the red or blue shift should be given. Take a overlay spectrum with reference at the background with different concentrations of sample in question. Check for the variation in bandwidth and PWHM to justify better.
Rf is an essencial identyf. value obtained by TLC you should provide it.
Regards
G
A accurately calculated Rf value is very important in TLC or HPTLC experiments.
Thanks a lot but what if the standard was run along with the sample in the same TLC plate n both the standard and the compound is showing the same florescent band
Ok. If that is the case, then Are you able to calculate the Rf value of the standard ? If the answer is affirmative, then hopefully you got the riposte.
I don't understand why you would not calculate the Rf value. As has been mentioned above, it is a distinguishing characteristic of your analyte along with its fluorescence, UV absorbance etc etc.
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