Can we collect scions from 1-year-old grafted plants instead of collecting scions from 40-year-old trees (candidate mother plant)? Are both plants physiologically and genetically the same? If yes, can we go for invitro shoot tip grafting (STG) by using scions from the grafted plants?
Dear Madhusmita Borah if the grafted plants mantain the genetic identity regarding the donor plants, there's not any problem: vegetative reproduction usually don't drive to the appearing of mutations, at least chimeric tissues are isolated, besides scions neither have any influence on this regard. A different thing is the physiollogical state. The age of donor plants usually has a great infleunce on the results of grafting: the younger the donor plants, the higher the success of grafting. Other question that you must to consider is the epigenetic state of donor plants, since important phenotypic variations can be observed, but these're no caused by genetic events.
Madhusmita Borah Please, consider a possible latent pathogen presence in the young grafted plants. The old mother plant is supposed to pass several tests for viruses and bacteria before collection of scions.
They are genetic ally identical but they may differs phsyologically. Although they we're, I suppose no problem at all..
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