Is it necessary to add epidermal growth factor to primary rat hepatocytes?

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How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to preserve the different plant tissues for seconadry metabolite estimation and other bioassays ?

Greetings, I am a PhD Scholar, I want to analyze the metabolites present in different tissues of bitter melon plant (Momordica charantia). I have grown the plants and I have the plant sample ready...

28 August 2023 764 2 View

How to find exchange current density (i₀) for volcano plot from DFT for HER?

In many articles, the exchange current (i₀) value for the volcano plot can be found from the Norskov's assumption. When I used their values, I couldn't achieve their results. The exchange current...

08 August 2023 6,641 0 View

FOXP3 Gene Mutations: Statistical Analysis of Healthy Volunteers and Diseased Patients?

Dear experts, We have analyzed the FOXP3 gene mutation of 10 healthy volunteers and 13 diseased samples. Out of these, 3 healthy volunteers (30%) and 8 diseased patients (61.53%) were found to...

21 July 2023 1,365 2 View

How to synthesis high molecular weight polyacrylamide?

I want to synthesize high molecular-weight polyacrylamide. I tried synthesising PAM using acrylamide, 2-acrylamido-2-methylpropane sulfonic acid in alkaline median like ph 7-8. In this case, I...

13 June 2023 2,086 4 View

How to Do Statistical Analysis of a Dataset: Smokers Protected from Diseases?

Dear Expert, We have a single group of data consisting of 520 volunteers. All of them were smokers and protected from certain diseases. In such a scenario, we would appreciate your guidance on how...

04 June 2023 8,802 5 View

Volume of distribution of Ascorbic acid in human?

Dear experts, Our team is working on a new intervention for drug dependence and we require information on the volume of distribution (Vd) for ascorbic acid in humans for our project. Would you...

08 April 2023 1,259 0 View

Setting the oral dose based on pharmacokinetic parameters?

Dear Kinetic experts, I need one kind suggestion from you. One X compound with molecular weight of 200.1, is highly water soluble with oral bio-availability of 65-75%. In that case I want to...

02 February 2023 9,696 4 View

Co-creation of customer experience and consumer experience: is it possible?

The co-creation of customer experience and consumer experience. Is it possible to create a theory development on this concept? Please shower your suggestions on

17 February 2022 8,952 4 View

How to mask nitrobenzene odor?

I want to prepare odourless nitrobenzene can explain how to do it? what are the chemicals used to do that?

06 February 2022 9,421 0 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

Good day!! All, can anyone recommend the best battery related journals to download research articles please?

I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...

01 August 2024 6,516 2 View

About impact factor?

I have been publishing the Nepalese Journal of Agricultural Sciences (online ISSN 2091-0428; print ISSN 2091-042X) on www.nepjas.com and research gate. I was wondering how I can get an impact...

01 August 2024 1,277 1 View

Sagnik Chatterjee

Sandwich between two layers of gelled collagen..till day-4/5

Adam Farmer

Absolutely not. As Sagnik said, collagen plates with an ecm overlay (after 6 hours in culture), plus the proper additives to your media. I suggest a Williams E media base, with dexamethasone, insulin, and l-glutamine.

Arumugam Suyavaran

Thanks a lot for your suggestions.