If not what are the alternatives. Culturing with Insulin and Glucagon seems to be inadequate for longevity of cells.
Sandwich between two layers of gelled collagen..till day-4/5
Absolutely not. As Sagnik said, collagen plates with an ecm overlay (after 6 hours in culture), plus the proper additives to your media. I suggest a Williams E media base, with dexamethasone, insulin, and l-glutamine.
Thanks a lot for your suggestions.
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