I have to block thiol groups present on the surface of a HLA antigen, can anyone recommend a non-toxic blocker soluble in water?

More Saurabh Pandit's questions See All

How can I perform RNA isolation from MRSA without using a kit?

I am trying to isolate RNA from Trizol and triton x but results are not coming, so help me out. Thanks

07 August 2014 6,589 5 View

How do I know if I have a problem with my primers or PCR?

I have taken HLA B27 specific primers from literature and tried to amplify them on DNA of an arthritis patient and there was no amplification. There was no amplification, i want to know that...

03 April 2014 9,379 12 View

Do anybody have an HPLC method for the detection of Cortisol in hairs?

Please share if you have.

03 April 2014 2,133 3 View

Is it necessary that if we have HLA B27 specific primer, it will amplify in a normal individual manner?

If yes why , please describe briefly.

03 April 2014 4,349 0 View

Can anyone help with my hemolysis problem?

I did hemolysis with my peptide at different concentration and it was observed that at high concentration the hemolysis was suppressed as compared to low concentration. I am not getting any...

03 April 2014 7,511 0 View

Which antigen i.e O, H or Vi should be taken to identify typhoid and why?

I am not able to find out the antigen specific for typhoid detection.

03 April 2014 341 1 View

How can we identify HLA present on the klebsiella residing in the human body?

Literature shows that klebsiella is also playing a part in arthritis, so can we identify klebsiella HLA by amplyfying with the primers use to amplify HLA on human PBMC.

03 April 2014 5,631 16 View

Does anyone know where I can get inhibitors of HLA b27, which are soluble in water?

Thanks.

02 March 2014 7,717 2 View

Can someone suggest a cELISA protocol?

For expression studies.

02 March 2014 6,133 5 View

Can anyone help with PBMC from whole blood by Ficoll method, I dissolved the pellets in PBS?

Viability was 57%, but how do I know that the cells shown are viable PBMC cells and as these are stored in PBS can I directly add a lysis buffer to extract DNA from these PBMC stored in PBS?

02 March 2014 5,520 4 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View