Is it immunoprecipitation band shifting?

Oki Jung @Oki-Jung

25 February 2017 1 5K Report

I am trying immunoprecipitation by protein G.

I tagged antibody( Rabbit) and I western blot using interesting protein antibody(mouse)

I got a result lower picture. but it looks a little shifting.

The size is 53kDa. so I am so worried that it could be a heavy chain.

Could you give me some advice?

1-lysate/2-tagged/ negative control

Oki Jung

Dear Alim seit-Nebi

Thank you for the answer. Thanks to your explain, I could understand why it has shift.:P

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