Oki Jung

4 Questions 10 Answers 0 Followers

Questions related from Oki Jung

Can I say it is one peak in qPCR melting curve?

I am doing qPCR and there are peak in NTC. (All samples are NTC) The values of NTCs were 28~30s I couldn't figure out what it is wrong. I changed primer, D.W, qPCR reagenrt and tried to use qPCR...

30 March 2017 402 7 View

Is it immunoprecipitation band shifting?

Hi I am trying immunoprecipitation by protein G. I tagged antibody( Rabbit) and I western blot using interesting protein antibody(mouse) I got a result lower picture. but it looks a little...

25 February 2017 5,138 1 View

How do i conclude negative control problem in chip assay?

hello. i am trying chip assay in manual. i tried to do immunoprecipitation by Protein A bead for chip. I set a negative control and target transcription factor. i used normal mouse antibody for...

06 February 2017 4,837 5 View

How to purify linear plasmid for transfection after enzyme cut?

hi i want to make stable cell. i use A549. i tried to do transfection on plasmid. but it is difficult to make it. so i tried to make linear plasmid before transfection. but i am not sure i have to...

07 August 2016 8,724 3 View