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Questions related from Oki Jung
I am doing qPCR and there are peak in NTC. (All samples are NTC) The values of NTCs were 28~30s I couldn't figure out what it is wrong. I changed primer, D.W, qPCR reagenrt and tried to use qPCR...
30 March 2017 397 7 View
Hi I am trying immunoprecipitation by protein G. I tagged antibody( Rabbit) and I western blot using interesting protein antibody(mouse) I got a result lower picture. but it looks a little...
25 February 2017 5,134 1 View
hello. i am trying chip assay in manual. i tried to do immunoprecipitation by Protein A bead for chip. I set a negative control and target transcription factor. i used normal mouse antibody for...
06 February 2017 4,832 5 View
hi i want to make stable cell. i use A549. i tried to do transfection on plasmid. but it is difficult to make it. so i tried to make linear plasmid before transfection. but i am not sure i have to...
07 August 2016 8,719 3 View