Hi all,

for a project, I would like to perform RBC Lysis of human whole blood and then put the cells in culture with PHA for 3 days prior to flow cytometry analysis, gating mainly on T lymphocytes.

We would like to avoid Ficoll isolation and stick with RBC Lysis.

Do you think this is feasible ? Or will our wells be polluted with too many platelets ?

Thank you.

Best regards.