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Questions related from Benjamin Tschumi
Hello, I generated human immature monocyte-derived DCs from CD14-monocytes using GM-CSF and IL-4 for 6 days. At D6, I see that all cells highly express CD209 (as compared to control monocytes)....
15 June 2021 9,998 6 View
Hello, I am trying to generate human Monocyte-derived Dendritic cells by culture of monocytes with GM-CSF and IL-4 for 6 days. Afterwards, I will use the TLR agonist R848 for 48h to induce DC...
20 April 2021 4,274 4 View
Hello, I would like to generate human moDCs for 6 days from CD14+ monocytes using IL-4+GM-CSF. At day 6, when I collect the floating cells, should I also consider the attached cells ? On day 6,...
16 April 2021 6,174 0 View
Hi all, I am having trouble with a cytotoxic assay : I plate a certain cell number in 96w plates. On the next day, I add serially diluted antibodies on the well and use AlamarBlue as a readout...
17 March 2021 7,867 0 View
Hi all, for a project, I would like to perform RBC Lysis of human whole blood and then put the cells in culture with PHA for 3 days prior to flow cytometry analysis, gating mainly on T...
11 June 2020 8,647 1 View
