Handling procedure of brain samples for RT-PCR / Western Blot?

29 February 2024 1 7K Report

Hello,

What would be the best procedure right after extracting brain samples from mice, if we plan to analyze the samples later ?

Would it be best to just directly flash freeze the brain tissue in isopentane before storing at -80°, or to homogenize and add some RNase or protease inhibitors before the freezing ?

Additionally, if the samples are sent to external collaborators for the analyses, what is the recommended temperature for shipping, is -20° cold enough ?

Many thanks,

Benjamin Vidal

Thank you very much Sebastian, interesting to know for the powder.

This is not for omics analyses but rather "simple", regular Western-Blot/RT-PCR (detection of a given transcript/protein).

I don't know if this makes a difference.

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