Protein expressed in Deuterated M9 Media goes into pellet ?

08 September 2018 9,493 8 View

Prodrg topology files, docking - can anyone help?

07 August 2014 5,027 5 View

Does anyone know the log-phase window for M9 media?

01 January 1970 8,747 0 View

BMRB submission of pdb structure obtained from cyana ?

My question is basically for someone who is familiar with CYANA software and have successfully submitted a pdb file calculated using NMR restraints using CYANA I am trying to execute the macro...

01 January 1970 2,785 0 View

Very slow cell growth in M9 media with a generation time of roughly 4hours?

I have a situation with bacterial growth in M9 media with two plasmidsWild Type (WT) and Mutant. BL21DE3-PLysS cells. procedure followed is as follows 1. Incoulated 5ml LB primary with a single...

01 January 1970 6,451 6 View

Cell clumps/floating debris found in primary M9 overnight culture?

I inoculate my 100ml M9 culture with cells from a 10ml MDAG - 135 media of 8 hours growth. After overnight incubation at 37degrees I see a good growth of the cells upto OD 2.0 but with also large...

01 January 1970 3,755 2 View

BL21DE3-PLysS cells give a slimy and sticky pellet...and have a pale wood yellow color?

BL21DE3-PLysS cells give a slimy and sticky pellet...and have a pale wood yellow color? Is it ok to have a slimy cell pellet? I suspect probably a few cells (may be around 10 % or so) lyse during...

01 January 1970 1,958 9 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View