Does anyone know the log-phase window for M9 media?

01 January 1970 0 9K Report

I ask this because if M9 media grows to a OD of 2 or 3 in the overnight culture are the cells in the log phase or stationary phase?

my cells (bl21de3-plysS) are finding a hard time to grow in the secondary M9 media of 1 liter hence I need to know if I am adding a good starter culture at proper OD to the secondary M9.

Badges
Science topic

More Chandrakala Gowda's questions See All

Protein expressed in Deuterated M9 Media goes into pellet ?

08 September 2018 9,493 8 View

Prodrg topology files, docking - can anyone help?

07 August 2014 5,027 5 View

BMRB submission of pdb structure obtained from cyana ?

My question is basically for someone who is familiar with CYANA software and have successfully submitted a pdb file calculated using NMR restraints using CYANA I am trying to execute the macro...

01 January 1970 2,785 0 View

Very slow cell growth in M9 media with a generation time of roughly 4hours?

I have a situation with bacterial growth in M9 media with two plasmidsWild Type (WT) and Mutant. BL21DE3-PLysS cells. procedure followed is as follows 1. Incoulated 5ml LB primary with a single...

01 January 1970 6,451 6 View

Is it essential to have a M9 primary?

I follow the following protocol to grow my bl21de3/bl21de3-plysS 1. 10ml of MDAG-135 primary media to be inoculated with a single colony. (6 to 9 hours upto O.D = 0.6 to 1.0) 2. 100ml M9 to which...

01 January 1970 2,308 2 View

Cell clumps/floating debris found in primary M9 overnight culture?

I inoculate my 100ml M9 culture with cells from a 10ml MDAG - 135 media of 8 hours growth. After overnight incubation at 37degrees I see a good growth of the cells upto OD 2.0 but with also large...

01 January 1970 3,755 2 View

BL21DE3-PLysS cells give a slimy and sticky pellet...and have a pale wood yellow color?

BL21DE3-PLysS cells give a slimy and sticky pellet...and have a pale wood yellow color? Is it ok to have a slimy cell pellet? I suspect probably a few cells (may be around 10 % or so) lyse during...

01 January 1970 1,958 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View