vaccinating through shaved abdominal region will be more beneficial. see the link

http://iai.asm.org/content/77/4/1514.long

Hi,

Dermis of mice ear has the most frequency of dendritic cells for triggering of immune response in comparison of all other body sites. But you should consider that the type of responses you needed is which one, Th1 or Th2. So, for optimizing of the route of immunization you should inject vaccine candidate at the different sites and compare the immunogenicity and potency.

In the past, response of DNA vaccines in mice and humans were very different. You have to be very careful in putting too many resources in doing studies in mice, unless you are developing a vaccine for mice (most unlikely).

You should select a site of injection in mice which should have similar vascularity as the proposed site for injection in humans. As mentioned above by Mehdi Mahdavi, presence of large number of dendritic cells in mice ear can have a significant impact on the quality and quantity of immune response.

For intradermal injections on the abdominal region of mice, you may not need to shave the region as hair are thin and you could easily inject intradermally in the abdominal region of mice by wetting the skin.

The link given by Mohammad Asad does not use intradermal as one of the routes for immunization. DTH has been evaluated by ID injection of mouse foot pad in the paper at the link.

Dendritic cells are efficient triggerer of immune response and their concentration is high in the dermis of the ear of mice which affects positively both the quality as well as quantity of immune response. 

Wetting of skin is sufficient to inject intradermally in the abdominal region of mice. 

However, for optimization of the route of immunization vaccine candidate must be injected at different sites followed by comparison of immunogenicity as well as potency.

Last but no the least vaccinating through shaved abdominal region in BALB/c mice help in eliciting protective immunity against certain protozoal (Leishmania  donovani) infection.

Thanks guys, I really appreciate the time you have given for these responses. We have being delivering DNA vaccines via the ear and I think that is what we will stick to. I just asked this to see whether it will be worthwhile comparing the two vaccination strategies. All the best to all of you with your research!

Hello,

an important issue in my opinion is the site also for the induction of mucosal immune response. A vaccination in the head region could putatively be more effective in inducing also a galt immune response, due to the drainage of antigens by cervical lymphnodes, while a vaccination in the abdomin al area could be more beneficial in eliciting a reproductive malt immune repsonse.

regards

Carlo

Hi Carlo, This is very interesting. Do you have a reference to support this (i.e. comparison between the two sites) or is this based on your personal experiences?

Hi

we assesses the mucosal response (Humoral)  to a vaccine in pigs (gut- published results) and female genital tract (unpublished), then making a few comparisons with references

Differential Blood and Mucosal Immune Responses

against an HIV-1 Vaccine Administered Via Inguinal or

Deltoid Injection

I started to think that the compartimantalization is due  to the stimulation of different linphonodal district of different compartments, and the recruitment of immune cell with a specific homing is the consequence. The mucosa compartments are most primitive, then is more logic, in my opinion, to adopt a local or locoregional point of view.

Bye

Hi Danuskha,

WIll it be possible for you to share the protocol of injecting into mice ear dermis? Actually, I want to iject Mtb for my research and we are finding difficult to ijnect in mice ear dermis.

Thanks

Amit Singh, I found this reference for intradermal injection of mouse ear. https://link.springer.com/protocol/10.1007%2F978-1-61779-876-4_9