7 Questions 28 Answers 0 Followers
Questions related from Mohammad Asad
Hi, I have a liposome of size 200-300 nm. If it will be labelled with fluorescence antibody, whether it can be studied through FACS? It is stable for FACS studies and we have LSR FORTESSA (BD) and...
08 February 2018 3,069 2 View
Hi, For a multicolored flow Cytometry experiment, we need to perform compensation of spectral overlaps (spill-over) due to usage of fluorochromes whose emission spectrum overlaps with each other....
01 March 2017 6,663 4 View
Dear all, Hello! How the size of individual cell can be measured by a typical SSC vs FSC graph without using a reference (known size beads or cells)? Although from a typical SSC vs FSC graph, we...
06 February 2017 5,746 5 View
In some papers they sorted the cells after intracellular staining for intracellular marker such as FOXP3 (as Treg marker). But for intracellular staining, they need to fix and permeablise the...
01 March 2016 3,721 3 View
In BALB/c mice splenocytes, from CD4+ T cells or CD8+ T cells, whether it is possible to obtain a CD56 positive cell population also? and if so than what could be its function, especially post...
13 January 2016 136 0 View
Hi, I am studying purified mice CD4 T cells, CD8 T cells and DCs etc proliferations in vitro labelled with CFSE. But total splenocyes are NOT showing proliferation. How can I observe splenocytes...
30 November 2015 5,432 11 View
While knocking out a gene from the mice, some un-calculated stressed must have been developed in the mice (for sure) due to the process of knock out. In that situation, how much it is honest and...
09 October 2015 3,738 0 View
