Mohammad Asad

10 Questions 32 Answers 0 Followers

Questions related from Mohammad Asad

What is the smallest sized particle which can be studied through FACS?

Hi, I have a liposome of size 200-300 nm. If it will be labelled with fluorescence antibody, whether it can be studied through FACS? It is stable for FACS studies and we have LSR FORTESSA (BD) and...

08 February 2018 3,120 2 View

Among cell of interest or compensation beads-Which of them is better for compensation in a FACS experiment?

Hi, For a multicolored flow Cytometry experiment, we need to perform compensation of spectral overlaps (spill-over) due to usage of fluorochromes whose emission spectrum overlaps with each other....

01 March 2017 6,697 4 View

How can we measure the size of a cell by using Flow Cytometer?

Dear all, Hello! How the size of individual cell can be measured by a typical SSC vs FSC graph without using a reference (known size beads or cells)? Although from a typical SSC vs FSC graph, we...

06 February 2017 5,803 5 View

How the cells sorted through FACS post intracellular staining for transcription factor etc can be used for functional study (say through co-culture)?

In some papers they sorted the cells after intracellular staining for intracellular marker such as FOXP3 (as Treg marker). But for intracellular staining, they need to fix and permeablise the...

01 March 2016 3,754 3 View

In BALB/c mice, can a CD56+ cell population be obtained from CD4+ T cells and CD8+ T cells?

In BALB/c mice splenocytes, from CD4+ T cells or CD8+ T cells, whether it is possible to obtain a CD56 positive cell population also? and if so than what could be its function, especially post...

13 January 2016 174 0 View

Will total mice splenocytes proliferate in in-vitro culture?

Hi, I am studying purified mice CD4 T cells, CD8 T cells and DCs etc proliferations in vitro labelled with CFSE. But total splenocyes are NOT showing proliferation. How can I observe splenocytes...

30 November 2015 5,477 11 View

Can I use anti-IgG whole molecule as isotype control in place of IgG sub classes for in-vivo neutralization experiments on mice?

Hello, I need to perform some in-vivo neutralization (by anti-body) experiments on BALB/c mice. My various experimental groups need different IgG sub-classes as isotype control. So, can I use IgG...

10 November 2015 4,023 0 View

How can I choose proper control for the Knock out mice?

While knocking out a gene from the mice, some un-calculated stressed must have been developed in the mice (for sure) due to the process of knock out. In that situation, how much it is honest and...

09 October 2015 3,784 0 View

Can I use soluble anti-CD3 and anti-CD28 for mouse regulatory T cell proliferation?

I want to see the proliferation of mouse Treg cells in co-culture. Can I use soluble anti-CD3 and anti-CD28 antibodies (in place of plat bound or bead based antibodies) for Treg proliferation? and...

07 August 2015 6,315 4 View

What should be the doses and time gap for blocking/neutralizing IL-10 and TGF-beta simultaneously/individually in BALB/c mice?

Hi, What doses of anti-IL-10 and anti-TGF-beta neutralizing antibodies I should administer in BALB/c mice for blocking IL-10 and TGF-beta cytokines respectively? How many doses should be...

26 May 2015 4,751 1 View