Hi, what is the possibility of a software that can freely download to draw a kite diagramme?

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How reducing sugar fluorescence assay chemistry work?

Hello. Can someone please explain the chemistry behind the assay in the following paper? Thank you RW https://www.sciencedirect.com/science/article/abs/pii/0003269789900043?via%3Dihub

27 September 2023 6,160 0 View

Where to find genomic DNA from Vibrio spp.?

Hi I'm looking to obtain genomic DNA from several vibrio species (excluding cholrae), for cloning purposes. Any recommendations on where I can get this material (besides ATCC)? Specifically...

21 September 2023 3,316 0 View

Can road humps be used to discharge traffic with constant headways (or at regular intervals) in heavy traffic flow situations? Any studies known?

Are there any studies done regarding this phenomenon?

01 September 2021 9,383 3 View

Why is my protein precipitating after isolation?

My protein is 28kda and is some what hydrophobic with a pI of 6 and has a his-tag. We typically purify in 1x TBS with low salt at pH 7.5. During dialysis and or concentration the protein...

18 September 2020 6,646 11 View

How to perform colony count in wound swabs?

I am planning to perform a colony count using the serial dilution methods on wound swabs transported in Amies transport medium. Could anybody please recommend a protocol to the above?

07 February 2018 3,943 1 View

What is the best way to transition media for hiPS cell culture?

Hello I have human iPS cells that are being thawed and cultured in mTeSR1 media. I want to transition them to E8 media. In the past we have had very limited success with this type of media...

31 October 2017 1,878 4 View

Is it better to do intradermal injections using the ear or shaved abdominal/thigh region of mice?

Dear all, We deliver DNA vaccines into the dermis of mice ears routinely, but I wonder whether delivering intradermal injections to shaved abdominal or thigh regions would be more immunogenic. I...

25 May 2015 2,495 10 View

What is the correct method/formula to calculate the p-value for qPCR data?

Please explain how the p-value and SD is calculated using Cp values.

15 July 2014 7,937 3 View

How reducing sugar fluorescence assay chemistry work?

Hello. Can someone please explain the chemistry behind the assay in the following paper? Thank you RW https://www.sciencedirect.com/science/article/abs/pii/0003269789900043?via%3Dihub

01 January 1970 1,868 0 View

Where to find genomic DNA from Vibrio spp.?

Hi I'm looking to obtain genomic DNA from several vibrio species (excluding cholrae), for cloning purposes. Any recommendations on where I can get this material (besides ATCC)? Specifically...

01 January 1970 3,523 0 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

How to make overlapping p-h diagram in EES?

I want to draw ph diagram using Engineering equation solver EES for refrigerant R134a and R1234fy and some other. I don't know how to draw the multiple ph diagram on same dome or overlapping to...

30 July 2024 647 7 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

How to prepare a molecule for molecular dynamics calculations?

If I want to calculate molecular dynamics (Gromacs), do I first need to optimize the geometry of the molecule? For example, I first draw the molecule in Avogadro, and then what? I guess I can't...

28 July 2024 673 3 View

What are the modern approaches to the organization and sustainable management of greenery in urbanized cities?

What are the modern approaches to the organization and sustainable management of greenery in urbanized cities? How can ecological and recreational functions be introduced into the landscape and...

23 July 2024 7,292 4 View

Is it possible to run the AIMD within a system using virtual crystal approximation (VCA)?

I want to study the thermal properties of a mixed system which is constructed by virtual crystal approximation in VASP. When I try to run the ab initio Molecular Dynamics of this system in VASP, I...

19 July 2024 6,569 3 View

Is it possible to estimate population using animal footprint?

I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...

18 July 2024 3,729 7 View