Is it beneficial to treat the RNA sample isolated by trizol method having low 260/280 ratio with DNAs I enzyme?

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Lata Israni Shukla

please check pH is it home made trizol

Sharmin Siddique Bhuiyan

Salam.

Yes. Always do a DNse I treatment after RNA extraction to get rid of any DNA that is contaminating my RNA sample. It will help you giving a meticulous concentration of RNA which might gives a high read during concentration check due to unwanted incorporation of DNA. Best wishes!

Sarker Mohammed Ibrahim Khalil

I have also same kind of problem. DNAse could be an option for you,