Sarker Mohammed Ibrahim Khalil

6 Questions 5 Answers 0 Followers

Questions related from Sarker Mohammed Ibrahim Khalil

Negative (-) cDNA concentration

Dear Colleagues and Mentors, I'm trying to quantify the relative expression of some genes. To quantify the gene expression, I'm planning to use cDNA concentration to normalize the ct values rather...

11 August 2017 8,114 0 View

Need help to sequence a partially sequenced gene.

Dear Colleagues and Mentors, Hope you all are doing fine. I'm trying to sequence a gene which has partially sequenced before. Now, our plan is extend the sequence or if possible get the whole...

24 June 2017 8,731 4 View

I am currently working with 35 SNPs from 11 samples ?

For the analysis, I'm using Geneclass2, Genepop, PGD Spider, Structure, Pophelper (online), Bayescan, Treeview, Arlequin. But i'm facing problem with the PGD Spider as it needs java. Could anybody...

01 May 2016 729 3 View

Can anyone help me to get some paper related to formalin?

1. Effects of formalin on fish. 2. How it affects to consumer? 3. Unethical use of formalin in fish market.

13 April 2015 2,989 7 View

Standard curve mystery.

Dear Colleagues and Mentors, I'm trying to determine the expression of some Genes with SYBR Green Quantitative RT-PCR protocols. But, I'm facing problems to formulate the standard curve. I tried...

01 January 1970 2,752 2 View

House keeping gene VS cDNA concentration.

Dear Colleagues and Mentors, Which one is better to follow to measure the expression of gene? Keeping one/set of house keeping gene(s) or direct measure of cDNA concentrations to normalize the...

01 January 1970 2,580 1 View