Dilara Herdem

3 Questions 1 Answers 0 Followers

Questions related from Dilara Herdem

Is there a sepharose FF column that you recommend to use in the purification of small proteins such as 3-5 kDa?

I am working with a recombinant peptide with a low molecular weight of 5 kDa. I want to use a sepharose FF column to purify this peptide. But the columns I found are used for peptides with large...

06 April 2023 7,209 3 View

Is imidazole autoclavable?

I will treat my protein samples with resin, for this I need to prepare imidazole. Is Sigma imidazole 56750 autoclavable?

05 January 2023 2,736 0 View

Which buffer do you use to remove salt from proteins in dialysis?

I precipitate the proteins with ammonium sulfate. I need to remove salt from samples. What can I use as a buffer in dialysis?

01 January 2023 1,127 1 View