What could be the reason for observing a band in NTC in RTPCR?

We have been getting Ct values almost similar to a test sample in NTC wells. The melt curve also overlaps with the test samples. While running a gel, a band with a product size similar to the...

02 March 2017 9,806 3 View

Can I reuse the calcein labelled tissue within PMMA blocks for decalcification and perform routine histology?

I will be tracking the bone velocity in vivo using Calcein injection in animals. I understand that the sections should be fixed in 70% ethanol before embedding them in PMMA to get the sections....

02 March 2016 8,567 9 View

What is the best way to transport RNA samples abroad?

09 October 2014 1,722 3 View

Can anyone help with GFP expression within cells?

I had recently encountered a strange problem while imaging using the confocal. The cells labelled with GFP (lentiviral) were transplanted into the animal. After 2 months, the animal was sacrificed...

05 June 2014 7,548 6 View

To what extent would the number of passage alter the miRNA expression?

There are many studies reporting the alteration in miRNA expression in the long-term cultures. But will there be any significant changes even when P2/P3 MSC cells are used for differentiation...

04 May 2014 5,730 1 View

Is there a possibility to screen the signaling molecules within bone marrow filtrate?

How to study the presence of a particular pathway in bone marrow filtrate?

02 March 2014 9,977 1 View

How to express RT-PCR data as percentage?

Usually RT PCR data is interpreted as fold increase but how do we express them as a percentage increase or decrease? Is there any formula or just multiply the fold increase by 100?

08 September 2013 4,279 0 View

GFP labelling of cells?

For GFP labelling, I am using P201, VSV-G and Delta 8.9 for the transfection along with PEI in the HEK 293T cells. I used the Qiagen kit for the isolation of plasmids as per the protocol. The...

06 July 2013 2,602 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View