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Questions related from Sowmya Ramesh
We have been getting Ct values almost similar to a test sample in NTC wells. The melt curve also overlaps with the test samples. While running a gel, a band with a product size similar to the...
03 March 2017 9,880 3 View
I will be tracking the bone velocity in vivo using Calcein injection in animals. I understand that the sections should be fixed in 70% ethanol before embedding them in PMMA to get the sections....
03 March 2016 8,628 9 View
Is there any other way apart from dry ice shipment that will maintain the integrity of the RNA samples?
10 October 2014 1,767 3 View
I was pelleting the cells which were transduced with Cop-GFP and the cell pellet appeared fluorescent green.
08 August 2014 8,909 4 View
I had recently encountered a strange problem while imaging using the confocal. The cells labelled with GFP (lentiviral) were transplanted into the animal. After 2 months, the animal was sacrificed...
06 June 2014 7,589 6 View
There are many studies reporting the alteration in miRNA expression in the long-term cultures. But will there be any significant changes even when P2/P3 MSC cells are used for differentiation...
05 May 2014 5,762 1 View
How to study the presence of a particular pathway in bone marrow filtrate?
03 March 2014 10,009 1 View
Usually RT PCR data is interpreted as fold increase but how do we express them as a percentage increase or decrease? Is there any formula or just multiply the fold increase by 100?
09 September 2013 4,319 0 View
For GFP labelling, I am using P201, VSV-G and Delta 8.9 for the transfection along with PEI in the HEK 293T cells. I used the Qiagen kit for the isolation of plasmids as per the protocol. The...
07 July 2013 2,688 1 View
