How to study the presence of a particular pathway in bone marrow filtrate?
This is a very broad question and the method depends on what you mean by "signalling molecules". If you are talking about intracellular proteins, you can try to permeabilize and fix bone marrow filtrate and do intracellular flow cytometry.
We have been getting Ct values almost similar to a test sample in NTC wells. The melt curve also overlaps with the test samples. While running a gel, a band with a product size similar to the...
02 March 2017 9,913 3 View
I will be tracking the bone velocity in vivo using Calcein injection in animals. I understand that the sections should be fixed in 70% ethanol before embedding them in PMMA to get the sections....
02 March 2016 8,660 9 View
Is there any other way apart from dry ice shipment that will maintain the integrity of the RNA samples?
09 October 2014 1,788 3 View
I was pelleting the cells which were transduced with Cop-GFP and the cell pellet appeared fluorescent green.
07 August 2014 8,938 4 View
I had recently encountered a strange problem while imaging using the confocal. The cells labelled with GFP (lentiviral) were transplanted into the animal. After 2 months, the animal was sacrificed...
05 June 2014 7,611 6 View
There are many studies reporting the alteration in miRNA expression in the long-term cultures. But will there be any significant changes even when P2/P3 MSC cells are used for differentiation...
04 May 2014 5,786 1 View
Usually RT PCR data is interpreted as fold increase but how do we express them as a percentage increase or decrease? Is there any formula or just multiply the fold increase by 100?
08 September 2013 4,383 0 View
For GFP labelling, I am using P201, VSV-G and Delta 8.9 for the transfection along with PEI in the HEK 293T cells. I used the Qiagen kit for the isolation of plasmids as per the protocol. The...
06 July 2013 2,715 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View