Usually RT PCR data is interpreted as fold increase but how do we express them as a percentage increase or decrease? Is there any formula or just multiply the fold increase by 100?
We have been getting Ct values almost similar to a test sample in NTC wells. The melt curve also overlaps with the test samples. While running a gel, a band with a product size similar to the...
02 March 2017 9,913 3 View
I will be tracking the bone velocity in vivo using Calcein injection in animals. I understand that the sections should be fixed in 70% ethanol before embedding them in PMMA to get the sections....
02 March 2016 8,660 9 View
Is there any other way apart from dry ice shipment that will maintain the integrity of the RNA samples?
09 October 2014 1,788 3 View
I was pelleting the cells which were transduced with Cop-GFP and the cell pellet appeared fluorescent green.
07 August 2014 8,938 4 View
I had recently encountered a strange problem while imaging using the confocal. The cells labelled with GFP (lentiviral) were transplanted into the animal. After 2 months, the animal was sacrificed...
05 June 2014 7,611 6 View
There are many studies reporting the alteration in miRNA expression in the long-term cultures. But will there be any significant changes even when P2/P3 MSC cells are used for differentiation...
04 May 2014 5,786 1 View
How to study the presence of a particular pathway in bone marrow filtrate?
02 March 2014 10,032 1 View
For GFP labelling, I am using P201, VSV-G and Delta 8.9 for the transfection along with PEI in the HEK 293T cells. I used the Qiagen kit for the isolation of plasmids as per the protocol. The...
06 July 2013 2,715 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering
05 August 2024 1,660 0 View