Hi - I'm looking for this cell line, if you have any leads please let me know!
ATCC doesn't seem to have them, so I would probably go to the Firestone lab or to a cell repository right there at UC Berkeley.
I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...
18 October 2020 6,639 3 View
07 December 2019 7,502 6 View
Almost all membrane pore characterizing techniques cannot be used due to the mechanical instability of the flat membrane. I want to use deionized water flux (DI) and mathematical way to determine...
04 December 2019 3,850 2 View
A given polypeptide protein has side chains and terminal (N- and C-). For any modification or conjugation reaction which part involved in reactions (side chains or terminals). Thank you !!
17 October 2019 575 2 View
For many days enzyme experiment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced by new to keep activity of enzymes?
01 September 2019 7,619 4 View
In my understanding there are three main types of tetrazolium dyes that are used to determine dehydrogenase incluing MTT, INT, and TTC and for some reason i have encountered that MTT is rarely...
11 September 2018 3,590 2 View
Hi, I need urgently implemented code for a "distributed community detection algorithm" in java, python, matlab or .... Can anyone help me?
03 September 2018 4,469 3 View
Hi all, I am trying to clone a 750bp Age1/Xho1 fragment cut from a minipreped cDNA into another minipreped vector of 7.5Kbp (Amp) cut with the same enzymes. Insert and vector bands were cut and...
05 May 2018 9,946 12 View
What is the right journal to contact for publishing a survey paper in transportation?
05 March 2018 2,981 3 View
27 December 2017 4,524 3 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View