I am trying to investigate the global methylation with the kit Imprint Methylated DNA Quantification kit (Sigma-Aldrich). However, the results of all my samples were higher than my control. Thus, all samples showed more than 100% of methylation.

Is anyone with the same problem?

We tried different concentrations of DNA but we always found the same: DNA methylation was higher in our samples compared to the methylated control.

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