How low must be the annealing temp of the first rounds for overhang pcr?

The primers i've designed have 5' overhang, which will be used for gene in/del (based on homologous recombination in yeast). To amplify this fragment, the annealing temperature of the first rounds...

13 January 2019 4,582 2 View

Is there research on Graphene supported by scientific software?

Any research on Graphene being carried out through computational science?

03 October 2017 3,889 3 View

How can I couple shell in solid using LS-DYNA?

Hello everyone,Does anyone know how to make the coupling of a steel connector on concrete? I used Coinstraned_Lagrange_In_Solid to the bars, but it does not work with shell elements, can someone...

19 April 2015 6,496 4 View

Which is relation between frequency of power in non thermal plasmas and eficiency of functionalization at polymer surfaces?

08 April 2015 4,875 4 View

No title

Has the reagent itself allowed the entry of DAPI into the nucleus? I am using Annexin + PI staining and I could not permeabilize the cells previously. Thanks.

11 August 2013 1,195 11 View

Consumer perception about photovoltaic distributed generation and reality

What are the factors that most influence the decision to purchase a photovoltaic system? What do consumers think about photovoltaic distributed generation? Are consumers' thoughts about the...

01 January 1970 4,357 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View