I would like to transfect human embryonic stem cell differentiated cardiomyocytes with GFP plasmid. Based on the literature, it does seems like, these cells are relatively difficult to transfect. I am looking for any such suggestions or experiences with transfecting hESC-CM, and also suggestions regarding which transfection reagent suits best for transfecting these cells, would be truly helpful.
Many thanks for your suggestions or advice in advance, Reena
These threads on RG might be helpful:
https://www.researchgate.net/post/Why_are_embryonic_cardiomyocytes_so_hard_to_transfect_with_non-viral_transfection_agents2
https://www.researchgate.net/post/Adult_cardiomyocyte_transfection2
https://www.researchgate.net/post/does_anyone_know_the_best_transfection_method_for_adult_rat_cardiomyocytes
A colleague of mine successfully used Viafect and Fugene for transfection of cardiomyocytes. Good luck!
The best is to try! Viromers give also very good results on cardiomyocytes.
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